mouse anti horse clone cvs22 isotype igg2a mca1086pe  (Bio-Rad)

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: MHC I expression on MSCs prior to and during co-culture with PBMCs

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: MHC II expression on MSCs prior to and during co-culture with PBMCs

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: B cell, T cell and CD8moderate/ CD25+ lymphocyte counts in co-culture at Day 3 ( a , c , e ) and Day 5 ( b , d , f ). B cell counts increased in PBMC only wells over time. Lower ratios of MSCs to PBMCs caused greater B cell counts. Significant differences between the co-culture groups are shown with brackets. Tregs were increased in 1:1 MSC/PBMC co-cultures as compared to cultures with a greater ratio of PBMCs. Blood donor co-cultures had consistently higher Tregs than other co-cultures. Total CD8moderate/CD25+ lymphocyte count illustrates elevated counts in MHC II-high co-cultures at Day 3 ( e ) and 5 ( f ). A representative sample of CD8moderate/ CD25+ lymphocytes is shown as the gated sample (black circle) ( g ). Significant differences between the co-culture groups are shown with brackets

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Co-Culture Assay

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: Neutrophil activation and MSC survival in co-culture with neutrophils at time points 6 h ( a , c ) and 12 h ( b , d ). Ratios of 1 MSC/1 neutrophil, 1MSC/10 neutrophils and 1 MSC/100 neutrophils and control MSCs (no neutrophils) are shown. At time 6 h, universal blood donor and MHC II-high co-cultures had ratios where activation was significantly greater than the autologous co-culture. At time 12 h, only the MCH II-high co-cultures had greater activation as compared to the universal blood donor (BD) co-cultures. Median MSC survival was greater than 95% when co-cultured with neutrophils at time point 6 h ( c ) and 12 h ( d ). The only group with significant viability loss with neutrophils as compared to MSCs cultured alone was the MHC II-high group at 12 h

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Activation Assay, Co-Culture Assay, Cell Culture

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: Anabolic gene expression in MSCs co-cultured with PBMCs

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: Catabolic gene expression in MSCs co-cultured with PBMCs

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Immune response to allogeneic equine mesenchymal stromal cells

doi: 10.1186/s13287-021-02624-y

Figure Lengend Snippet: PBMC proliferation in co-culture with MSCs at Day 3 ( a ) and Day 5 ( b ). MHC II-high MSCs caused significantly greater PBMC proliferation on Day 3 than the autologous group. Autologous MSCs caused significantly greater PBMC proliferation than the MHC II-low co-cultures at Day 5. Significant differences between the co-culture groups only are shown with brackets

Article Snippet: MSCs were stained for MHC I (CVS22, BioRad, Hercules, CA, USA) and MHC II (CVS20, BioRad, Hercules, CA, USA) using antibodies used in previous publications [ , ].

Techniques: Co-Culture Assay